Cleaved-Caspase-9 (D353) rabbit pAb

Cat :A12613

Caspase9,Caspase 9,A12613, 12613, CASP9, Caspase-9, CASP9; MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6

Price： ¥900¥1600

Size： 50ul 100ul

Datasheet

MSDS

Source

Rabbit
Applications

IF,WB,IHC,ELISA
Reactivity

Mouse,Rat
Dilution

IF: 1:50-200 WB: 1:500 - 1:2000. IHC: 1:100 - 1:300. ELISA: 1:20000. Not yet tested in other applications.
Storage

-20°C/1 year
Specificity

Cleaved-Caspase-9 (D353) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-9 protein resulting from cleavage adjacent to D353.
Source/Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen

The antiserum was produced against synthesized peptide derived from human Caspase 9. AA range:323-372
Alternative names

CASP9; MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6
Form

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality

Polyclonal
Isotype

IgG
Background

CASP9 encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Caspase 9 can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. Caspase 9 is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
Other

CASP9, Caspase-9
Mol.Wt (Da)

42975
Concentration

1 mg/ml