Cyclin D1 (phospho Thr286) rabbit pAb

Cat :A13237

Cyclin,A13237, 13237, CCND1, G1/S-specific cyclin-D1, CCND1; BCL1; PRAD1; G1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene

Price： ¥900¥1600

Size： 50ul 100ul

Datasheet

MSDS

Source

Rabbit
Applications

WB,IHC,ELISA
Reactivity

Human,Mouse,Rat
Dilution

WB: 1:500 - 1:2000. IHC: 1:100 - 1:300. ELISA: 1:10000. Not yet tested in other applications.
Storage

-20°C/1 year
Specificity

Phospho-Cyclin D1 (T286) Polyclonal Antibody detects endogenous levels of Cyclin D1 protein only when phosphorylated at T286.
Source/Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen

The antiserum was produced against synthesized peptide derived from human Cyclin D1 around the phosphorylation site of Thr286. AA range:246-295
Uniprot No

P24385
Alternative names

CCND1; BCL1; PRAD1; G1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene
Form

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality

Polyclonal
Isotype

IgG
Background

cyclin D1(CCND1) Homo sapiens The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008],
Other

CCND1, G1/S-specific cyclin-D1
Mol.Wt (Da)

33729
Concentration

1 mg/ml