Pan Methylated Lysine mouse mAb(Mix)

Cat :A19452

Methylated Lysine,A19452, 19452, Pan Methylated Lysine

Price： ¥900¥1600

Size： 50ul 100ul

Datasheet

MSDS

Source

Mouse
Applications

WB,IHC
Reactivity

Species independent
Dilution

WB: 1:1000-2000 IHC: 1:200-500
Storage

-20°C/1 year
Specificity

The antibody detects endogenous Pan Methylated Lysine protein.
Source/Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Immunogen

Conjugated Protein
Form

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Clonality

Monoclonal
Isotype

IgG
Background

Methylation of lysine residues is a common regulatory posttranslational modification (PTM) that results in the mono-, di-, or tri-methylation of lysine at ε-amine groups by protein lysine methyltransferases (PKMTs). Two PKMT groups are recognized based on structure and catalytic mechanism: class I methyltransferases or seven β strand enzymes, and SET domain-containing class V methyltransferases. Both use the methyl donor S-adenosyl-L-methionine to methylate histone and non-histone proteins. Class I methyltransferases methylate amino acids, DNA, and RNA. Six methyl-lysine-interacting protein families are distinguished based on binding domains: mBT, PHD finger, Tudor, PWWP, WD40 repeat, and chromodomains. Many of these display differential binding preferences based on lysine methylation state. KDM1 subfamily lysine demethylases catalyze demethylation of mono- and di-methyl lysines, while 2-oxoglutarate-dependent JmjC (KDM2-7) subfamily enzymes also modify tri-methyl lysine residues.
Concentration

1 mg/ml