Rad17 (phospho Ser645) rabbit pAb

Cat :A20494

Rad17 ,A20494, 20494, RAD17, Cell cycle checkpoint protein RAD17, RAD17; R24L; Cell cycle checkpoint protein RAD17; hRad17; RF-C/activator 1 homolog

Price： ¥900¥1600

Size： 50ul 100ul

Datasheet

MSDS

Source

Rabbit
Applications

WB,ELISA
Reactivity

Human,Mouse
Dilution

WB: 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.
Storage

-20°C/1 year
Specificity

Phospho-Rad17 (S645) Polyclonal Antibody detects endogenous levels of Rad17 protein only when phosphorylated at S645.
Source/Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen

The antiserum was produced against synthesized peptide derived from human RAD17 around the phosphorylation site of Ser645. AA range:621-670
Uniprot No

O75943
Alternative names

RAD17; R24L; Cell cycle checkpoint protein RAD17; hRad17; RF-C/activator 1 homolog
Form

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality

Polyclonal
Isotype

IgG
Background

RAD17 checkpoint clamp loader component(RAD17) Homo sapiens The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, h
Other

RAD17, Cell cycle checkpoint protein RAD17
Mol.Wt (Da)

77055
Concentration

1 mg/ml