Raf-1 (phospho Ser296) rabbit pAb

Cat :A20521

Raf-1,A20521, 20521, RAF1, RAF proto-oncogene serine/threonine-protein kinase, RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1

Price： ¥900¥1600

Size： 50ul 100ul

Datasheet

MSDS

Source

Rabbit
Applications

WB,IHC,ELISA
Reactivity

Human,Mouse,Rat
Dilution

WB: 1:500 - 1:2000. IHC: 1:100 - 1:300. ELISA: 1:20000. Not yet tested in other applications.
Storage

-20°C/1 year
Specificity

Phospho-Raf-1 (S296) Polyclonal Antibody detects endogenous levels of Raf-1 protein only when phosphorylated at S296.
Source/Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen

The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Ser296. AA range:271-320
Uniprot No

P04049
Alternative names

RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
Form

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality

Polyclonal
Isotype

IgG
Background

Raf-1 proto-oncogene, serine/threonine kinase(RAF1) Homo sapiens This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008],
Other

RAF1, RAF proto-oncogene serine/threonine-protein kinase
Mol.Wt (Da)

73052
Concentration

1 mg/ml