Separase rabbit pAb

Cat :A21383

Separase ,A21383, 21383, ESPL1, Separin, ESPL1; ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase

Price： ¥900¥1600

Size： 50ul 100ul

Datasheet

MSDS

Source

Rabbit
Applications

WB,IHC,IF,ELISA
Reactivity

Human,Mouse
Dilution

WB: 1:500 - 1:2000. IHC: 1:100 - 1:300. IF: 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
Storage

-20°C/1 year
Specificity

Separase Polyclonal Antibody detects endogenous levels of Separase protein.
Source/Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen

The antiserum was produced against synthesized peptide derived from human SEPARASE. AA range:767-816
Uniprot No

Q14674
Alternative names

ESPL1; ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase
Form

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality

Polyclonal
Isotype

IgG
Background

extra spindle pole bodies like 1, separase(ESPL1) Homo sapiens Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010],
Other

ESPL1, Separin
Mol.Wt (Da)

233113
Concentration

1 mg/ml