Caspase-3 rabbit pAb

Source

Rabbit

Applications

WB,IHC

Reactivity

Human,Chicken(testedbyourcustomer）

Dilution

WB: 1:1000-2000 IHC: 1:200-500

Storage

-20°C/1 year

Specificity

The antibody detects endogenous Caspase-3 protein.

Source/Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen

Recombinant Protein of Caspase-3

Uniprot No

P42574

Form

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Clonality

Polyclonal

Isotype

IgG

Background

caspase 3(CASP3) Homo sapiens This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008],

Other

Concentration

1 mg/ml