The specific activity of Akt1 was determined to be 125 nmol/min/mg using a synthetic peptide substrate (RPRAATF) (see activity assay protocol).
>90%, by densitometry
Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
Recombinant full-length human Akt1 was expressed by baculovirus in Sf 9 insect cells using an N-terminal GST tag. The gene accession number is HNM_005163H
Product | Reactivity | Applications | Conjugation | Catalog | Images |
---|